Powder: -20°C for 3 years | In solvent: -80°C for 1 year
Amsacrine hydrochloride (acridinyl anisidide hydrochloride) 是一种拓扑异构酶 II 抑制剂,用于治疗急性髓细胞性白血病。
规格 | 价格/CNY | 货期 | 数量 | |
---|---|---|---|---|
1 mg | ¥ 139 | 现货 | ||
5 mg | ¥ 297 | 现货 | ||
10 mg | ¥ 497 | 现货 | ||
25 mg | ¥ 693 | 现货 | ||
50 mg | ¥ 897 | 现货 | ||
100 mg | ¥ 1,230 | 现货 | ||
200 mg | ¥ 1,720 | 现货 | ||
500 mg | ¥ 2,660 | 现货 | ||
1 mL * 10 mM (in DMSO) | ¥ 327 | 现货 |
产品描述 | Amsacrine hydrochloride (acridinyl anisidide hydrochloride) is topoisomerase II inhibitor , is used in the treatment of acute myelogenous leukemia. |
体外活性 | Amsacrine hydrochloride blocked HERG currents in HEK 293 cells and Xenopus oocytes in a concentration-dependent manner, with IC50 values of 209.4 nm and 2.0 microm, respectively. HERG channels were primarily blocked in the open and inactivated states, and no additional voltage dependence was observed. Amsacrine caused a negative shift in the voltage dependence of both activation (-7.6 mV) and inactivation (-7.6 mV). HERG current block by amsacrine was not frequency dependent. The S6 domain mutations Y652A and F656A attenuated (Y652A) or abolished (F656A, Y652A/F656A) HERG current blockade, indicating that amsacrine binding requires a common drug receptor within the pore-S6 region[1]. |
体内活性 | In animals treated with different doses of amsacrine (0.5-12 mg kg(-1) ), the frequencies of micronucleated polychromatic erythrocytes increased significantly after treatment with 9 and 12 mg kg(-1) . caused significant suppressions of erythroblast proliferation at higher doses.amsacrine has high incidences of clastogenicity and low incidences of aneugenicity[2]. |
细胞实验 | Voltage-clamp measurements of Xenopus oocytes were performed in a solution containing (in mM): 5 KCl, 100 NaCl, 1.5. CaCl2, 2 MgCl2 and 10 HEPES (pH adjusted to 7.4 with NaOH). Current and voltage electrodes were filled with 3 m KCl solution.?For whole-cell patch-clamp recordings from HEK 293 cells, electrodes were filled with the following solution (in mM): 130 K-aspartate, 5.0 MgCl2, 5 EGTA, 4 ATP, 10 HEPES (pH adjusted to 7.2 with KOH). The external solution for these experiments contained (in mM): 137 NaCl, 4.0 KCl, 1.0 MgCl2, 1.8 CaCl2, 10 HEPES, 10 glucose (pH adjusted to 7.4 with NaOH).?Amsacrine was prepared as 10 mm stock solution in DMSO and stored at -20℃. On the day of experiments, aliquots of the stock solution were diluted to the desired concentrations with the bath solution. HERG current amplitudes (recorded from Xenopus oocytes) were not significantly altered upon application of 1% DMSO (v v^-1; maximum bath concentration) for 20 min. In addition, DMSO did not affect HERG channel currents recorded from HEK 293 cells at concentrations up to 0.3% (maximum bath concentration in this study: 0.1% DMSO)[1]. |
别名 | acridinyl anisidide hydrochloride, 盐酸胺苯吖啶, m-AMSA hydrochloride |
分子量 | 429.92 |
分子式 | C21H20ClN3O3S |
CAS No. | 54301-15-4 |
Powder: -20°C for 3 years | In solvent: -80°C for 1 year
DMSO: 4.3 mg/mL (10 mM)
可选溶剂 | 浓度 体积 质量 | 1 mg | 5 mg | 10 mg | 25 mg |
DMSO | 1 mM | 2.326 mL | 11.6301 mL | 23.2601 mL | 58.1504 mL |
5 mM | 0.4652 mL | 2.326 mL | 4.652 mL | 11.6301 mL | |
10 mM | 0.2326 mL | 1.163 mL | 2.326 mL | 5.815 mL |
对于不同动物的给药剂量换算,您也可以参考 更多...
请在以下方框中输入您的动物实验信息后点击计算,可以得到母液配置方法和体内配方的制备方法: 比如您的给药剂量是10 mg/kg,每只动物体重20 g,给药体积100 μL,一共给药动物10 只,您使用的配方为5% DMSO+30% PEG300+5% Tween 80+60% ddH2O。那么您的工作液浓度为2 mg/mL。
母液配置方法:2 mg 药物溶于 50 μL DMSO (母液浓度为 40 mg/mL), 如您需要配置的浓度超过该产品的溶解度,请先与我们联系。
体内配方的制备方法:取 50 μL DMSO 主液,加入 300 μL PEG300, 混匀澄清,再加 50 μL Tween 80,混匀澄清,再加 600 μL ddH2O, 混匀澄清。
您可能有的问题的答案可以在抑制剂处理说明中找到,包括如何准备库存溶液,如何存储产品,以及基于细胞的分析和动物实验需要特别注意的问题。
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